Description

Products Code: Terragene Bionova BT224 Steam Steam Ultra Rapid Biological Indicator (20min)

Usage: Temperature D-Value: Not lower than 1.5 minutes at 121 °C & Another D-value is informed at 132 °C and at 135 °C. Z-Value is informed. (Final fluorescence reading is performed after 20min incubation at 60°C)

Characteristics: Bionova Incubate the indicator for a maximum of 20min

Packaging: 1 Box (50 Pcs)

Shelf Life: 2 Years.

Storage conditions:  10-30 °C keep in a dark place in its original box.

Microorganism: Geobacillus Stearothermophilus spores per vial.

Regulations: IRAM 37102-1, IRAM 37102-3, ISO 11138-1, ISO 11138-3

Instructions for Use: After the sterilization process has finished, open the sterilizer door, wait five minutes and remove the SCBI from the package. Let the SCBI cool down until it reaches room temperature. A color change to brown indicates that the SCBI has been exposed to Steam. Press the lid to seal the tube. Crush the ampoule contained in the SCBI with an individual ampoule crusher or with the ampoule crusher placed within the incubator´s incubation area. Then shake the tube down vigorously, with movements similar to those performed to lower the temperature in a mercury thermometer, until the medium reaches the base of the tube and soaks the spore carrier entirely. Finally, place the SCBI in the incubator.

WARNING: Do not crush or handle the SCBI excessively, since this might cause the glass ampoule to burst. Let the SCBI cool down until it reaches room temperature.

IMPORTANT: This chemical indicator color change does not evidence the process effectiveness to achieve sterility. If the Process Indicator color has not changed, check the sterilization process.

Reminder: Incubate the processed indicator and the positive control indicator in the appropriate Bionova Auto-Reader Incubators for a maximum of 20 minutes at (60 ± 2) °C for ultra rapid readout.

Holding time between sterilization and incubation should not exceed a 7-day period. Fluorescence detection by the reader (excitation 340-380 nm / emission 455-465 nm) means a failure in the sterilization process. If no fluorescence is detected at 20-minute incubation, the result is negative. The positive control must give positive fluorescence readout. It is good practice to incubate a positive control for a visual color change.